Publications

  1. Kusnadi, A. R., Z. L. Nikolov, and C. Ford. 1993. Production of a functional starch-binding domain of Aspergillus glucoamylase-I in Escherichia coli. Gene 127:193.
  2. Dalmia, B. K. and Z. L. Nikolov. 1994. Characterization of a β-galactosidase fusion protein containing the starchbinding domain of Aspergillus glucoamylase. Enzyme Microb. Technol. 16:18.
  3. Cantarella, L., Z. L. Nikolov, and P. J. Reilly. 1994. Oligosaccharide production by glucoamylase in aqueous ether mixtures. Enzyme Microb. Technol. 16:383.
  4. Evangelista, R. L., A. J. Mangold, and Z. L. Nikolov. 1994. Recovery of lactic acid by sorption: Resin evaluation. Appl. Biochem. Biotechnol. 45/46:131.
  5. Dalmia, B. K. and Z. L. Nikolov. 1994. A glutathione S-transferase fusion protein with the starch-binding domain of Aspergillus glucoamylase. Ann. N.Y. Acad. Sci. 721:169.
  6. Dalmia, B. K., K. Schutte, and Z. L. Nikolov. 1995. Domain E of Bacillus macerans cyclodextrin glucanotransferase: An independent starch-binding domain. Biotechnol. Bioeng. 47: 575.
  7. Chen, L. Coutinho, P.M., Nikolov, Z. L., Ford, C. 1995. Deletion analysis of the starch-binding domain of Aspergillus glucoamylase. Protein Eng. 8: 1049-1-55.
  8. Evangelista, R. L. and Z. L. Nikolov. 1996. Recovery and purification of lactic acid from fermentation broth by adsorption. Appl. Biochem. Biotechnol. 57/58: 471.
  9. Kusnadi, A. R., J. Howard, and Z. L. Nikolov. 1997. Production of recombinant proteins in transgenic plants: Practical considerations. Biotechnol. Bioeng. 56: 473.
  10. Kusnadi, A. R., R. L. Evangelista, E. E. Hood, J. Howard, and Z. L. Nikolov. 1998. Production and purification of two recombinant proteins from transgenic corn. Biotechnol. Progress 14: 149-155.
  11. Ferreira, S. R., Z. L. Nikolov, L. K. Doraiswamy, M. A. Meireles, and A. J. Petenate. 1999. Supercritical fluid extraction of black pepper essential oil. J. Supercritical Fluids 14: 235-245.
  12. Kusnadi, A. R., R. L. Evangelista, E. E. Hood, J. Howard, and Z. L. Nikolov. 1998. Processing of transgenic corn and its effect on the recovery of β-glucuronidase. Biotechnol. Bioeng. 60: 44-52.
  13. Evangelista, R. L., A. R. Kusnadi, J. Howard, and Z. L. Nikolov. 1998. Process and economic evaluation of the recovery of recombinant β-glucuronidase from transgenic corn. Biotechnol. Progress. 14: 607-614.
  14. Chang, H.Y., Irwin, P.M., and Nikolov, Z.L. 1998. Effects of mutations in the starch-binding domain of Bacillus macerans cyclodextrin glycosyltransferase. J. Biotechnol. 65: 191-202.
  15. Bai, Y. and Nikolov, Z. L. 2001. Effect of processing on the recovery of recombinant β–glucuronidase from transgenic canola. Biotechnol. Progress 17:168-74.
  16. Yildirim, S., R. G. Fuentes, R. Evangelista, and Z.L. Nikolov, 2001. Fractionation of transgenic corn for optimal recovery of recombinant proteins, J. Am. Oil Chem. Soc., 79:809-814.
  17. Streatfield, S. J, J. M. Major, D. K. Barker, C. Brooks, S. L. Woodard, M. Horn, Z. L. Nikolov, E.E. Hood, J. M. Jilka, and J.A. Howard 2002. Development of and edible subunit vaccine in corn against enterotoxigenic strains of E. coli. In Vitro Cell. Dev. Biol. Plant. 38: 11-17.
  18. Azzoni A. R , A. R. Kusnadi; E. A. Miranda, and Z. L. Nikolov. 2002. Recombinant aprotinin produced in transgenic corn seed: Extraction and Purification Studies, Biotechnol. Bioeng., 80: 268-276.
  19. Bai, Y., Z. N. Nikolov, and C. E. Glatz. 2002. Aqueous extraction of β-glucuronidase from transgenic canola: Kinetics and microstructure, Biotechnol. Progress, 18:1301-1305.
  20. Menkhaus, T.J., Bai , Y., Zhang, C., Nikolov, Z.L., Glatz, C.E. 2004. Considerations for the recovery of recombinant proteins from plants, Biotechnol Prog., 20:1001-1004.2
  21. Nikolov, Z. L, .Woodard, S. 2004. Downstream processing of recombinant proteins from transgenic feedstock, Curr Opin Biotechnol., 15: 479-486.
  22. Lamphear, B. J., Barker, D. K. Brookes, C. A. Delaney, D. E., Lane, J. R. Beifuss, K, Love, R. Thompson, K. Mayor, J., Horn, M. E , Streatfield, S. J., Nikolov, Z., Woodard, S.L., Hood E. E., and Howard, J. A. 2005. Expression of the sweet protein brazzein in maize for product of a new commercial sweetener. Plant Biotechnol. J., 3: 103-114.
  23. Nandi, S, Yalda, E, Lu. S., Nikolov, Z.L, Misaki, R., Kazuhito, F, Huang N. 2005. Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain. Transgenic Res., 14: 237-249
  24. Azzoni A. R , K. Takahashi, S.L. Woodard; E. A. Miranda, Z. L. Nikolov. 2005. Purification of recombinant aprotinin produced in transgenic corn seed: Separation from CTI utilizing ion-exchange chromatography. J. Braz. J. Chem. Eng.: 22: 323-330.
  25. Zhu, L.; van de Lavoir, M. C.; Albanese J.; Beenhouwer, D. O.; Cardarelli, P.M.; Deng, D. F.; Deshpande S.; Diamond, J. H.; Green, L; Nikolov, Z. L. et al. 2005. Production of human monoclonal antibodies in eggs of chimeric chickens. Nat. Biotechnol., 23: 1159-1169.
  26. Wilken, L. R., and Nikolov, Z. L. 2006. Factors influencing recombinant human lysozyme extraction and cation exchange adsorption. Biotechnol. Prog., 22: 745-752.
  27. Wilken, L. and Nikolov, Z. L., 2006. Effect of rice extract impurities on cation exchange adsorption of lysozyme. ASABE Annual International Meeting, Paper # 067074

Book Chapters

  1. Nikolov, Z. L. and P. J. Reilly. Enzymatic Depolymerization of Starch in Biocatalysts for Industry (J. S. Dordick, ed.),Plenum, New York, 1991, pp. 37-62.
  2. Nikolov, Z. L., P. Maheshwari, J. E. Hardwick, P. A. Murphy, and L. A. Johnson. Potential Applications for Supercritical Carbon Dioxide Separations in Soybean Processing in Food Science and Human Nutrition (G. Charalambous, ed.), Elsevier, Amsterdam, 1992, pp. 595-616.
  3. Meireles, M. A. and Z. L. Nikolov. Extraction and Fractionation of Essential Oils with Liquid Carbon Dioxide. In: Herbs, Spices, and Edible Fungi (G. Charalambous, ed.), Elsevier, 1994, pp. 171-199.
  4. Nikolov, Z. L. and R. Fuentes-Granados, 1999. Soybeans (Fermentation, Meal, Oil) in The Encyclopedia of Bioprocess Technology, (Flickinger, M. and Drew, S., eds.), Wiley, New York.
  5. Nikolov, Z. L. and Hammes, D. Production of Recombinant Proteins from Transgenic Crops in Plants as Factories for Protein Production, (E.E. Hood and J. Howard, eds.) Kluwer Academic Publishers, Dordrecht, 2002, 159-174.
  6. Delaney, D., Jilka, J, Irwin, P. Nikolov, Z., Hood, E. and Howard, J. Production of Aprotinin in Transgenic Maize Seeds for the Pharmaceutical and Cell Culture Markets, in Plant Biotechnology 2002 and Beyond, (K. Vasil , ed.), Kluwer, 2003, pp. 393-394.
  7. Woodard, S. and Nikolov, Z. L., Bioseparation of Proteins Expressed in Plant/Animal Products in The Encyclopedia of Agricultural, Food and Biological Engineering, (D. Heldman, ed.), Taylor &Francis. 2008.
  8. Nikolov, Z. L. Regan, J. T., Dickey. L. F. and Woodard, S. L. Purification of Antibodies from Transgenic Plants in Process Scale Purification of Antibodies (Uwe Gottschalk, ed), Wiley, 2008, in press.